| Viral Smear Kit, Herpes etc. |
PURPOSE:
This kit can be picked up from Cap-Lab and describes collection and
transportation methods for smears from skin, oral and external
genital and miscellaneous mucosal lesions.
NOTE:
1.
In this kit, you are provided with 2 frosted-end slides in a cardboard
folder, a wooden spatula, a sterile polyester fibber-tipped swab, a
Cytology Non-Gyn form, and this set of instructions. Please also pick
up a bottle of Cytology spray fixative. A thin prep vial or sure path vial
for liquid collection is also available and is the preferred method of
collection.
2.
Skin and mucosal lesions may be scraped, smeared on a slide, fixed
immediately and sent to Cytology for the detection of viral (especially
Herpes) and fungal infections. (Kit is not intended for microbiology
culture, KOH or immunofluorscence)
3.
Viral inclusions and fungus are seen via routine pap stain, however,
certain fungal smears usually require special stains. Therefore, you
must request examination for virus or fungus on the form.
4.
The preferred method of collection for viral inclusions is to place the
collecting device in cytolyt and return to lab for processing. If
collection device does not have a snap off head than rotate in liquid
collection fixative clockwise and counterclockwise to remove all the
cellular specimen.
5.
Print the complete patient information on the form and include
insurance information. Complete the form for specimen type, date
and for clinical history and diagnosis. Be sure to include the exact
location and description of the lesion.
6.
Label all slides with patient’s name. Write on frosted side with pencil.
Extra slides in folders are provided.
7.
For lesions which appear dry, apply wet compress for ½ hour before
scraping. This not only makes the lesion softer for easier scraping, but
also removes degenerated debris.
8.
If the lesion is blistered, remove the dome and scrape the margins of
the ulcer or imprint on the slide. Visible fluid is usually unsatisfactory
because it rarely contains viable cells.
9.
After removal of the crust, the ulceration should be energetically
scraped with the wooden spatula or sterile swab. Use of spatula or
swab is personal preference, however, the spatula will probably yield
more cells.
10.
Scrapings should be immediately submersed in the cytolyt vial.
Rotations in a clockwise and counter clockwise directions are made.
Taking care to remove most of specimen into liquid fixative.
11.
If preferred method of collection cannot be done, scrapes should be
quickly smeared on one slide and immediately sprayed with Cytology
fixative. Two or three sprays are adequate. Hold spray bottle six inches
from the slide. Then proceed with the second slide.
12.
Please submit at least 2 slides from the lesion, if possible. When a
fungal lesion is suspected, one slide will be used for pap stain and
another for fungal stain.
13.
Allow fixed slides to dry completely before closing the cardboard slide
folder.
14.
Fold and wrap the Cytology form around the folder.
15.
Transport the smears and bottle of fixative directly to Cap-Lab
Cytology department. To arrange for transportation of specimens call
517-372-5520.
REFERENCE:
Naib, Zuher, M.D. : Exfoliative Cytopathology, Second Edition, Little,
Brown and Co., Boston, 1976.
This kit can be picked up from Cap-Lab and describes collection and
transportation methods for smears from skin, oral and external
genital and miscellaneous mucosal lesions.
NOTE:
1.
In this kit, you are provided with 2 frosted-end slides in a cardboard
folder, a wooden spatula, a sterile polyester fibber-tipped swab, a
Cytology Non-Gyn form, and this set of instructions. Please also pick
up a bottle of Cytology spray fixative. A thin prep vial or sure path vial
for liquid collection is also available and is the preferred method of
collection.
2.
Skin and mucosal lesions may be scraped, smeared on a slide, fixed
immediately and sent to Cytology for the detection of viral (especially
Herpes) and fungal infections. (Kit is not intended for microbiology
culture, KOH or immunofluorscence)
3.
Viral inclusions and fungus are seen via routine pap stain, however,
certain fungal smears usually require special stains. Therefore, you
must request examination for virus or fungus on the form.
4.
The preferred method of collection for viral inclusions is to place the
collecting device in cytolyt and return to lab for processing. If
collection device does not have a snap off head than rotate in liquid
collection fixative clockwise and counterclockwise to remove all the
cellular specimen.
5.
Print the complete patient information on the form and include
insurance information. Complete the form for specimen type, date
and for clinical history and diagnosis. Be sure to include the exact
location and description of the lesion.
6.
Label all slides with patient’s name. Write on frosted side with pencil.
Extra slides in folders are provided.
7.
For lesions which appear dry, apply wet compress for ½ hour before
scraping. This not only makes the lesion softer for easier scraping, but
also removes degenerated debris.
8.
If the lesion is blistered, remove the dome and scrape the margins of
the ulcer or imprint on the slide. Visible fluid is usually unsatisfactory
because it rarely contains viable cells.
9.
After removal of the crust, the ulceration should be energetically
scraped with the wooden spatula or sterile swab. Use of spatula or
swab is personal preference, however, the spatula will probably yield
more cells.
10.
Scrapings should be immediately submersed in the cytolyt vial.
Rotations in a clockwise and counter clockwise directions are made.
Taking care to remove most of specimen into liquid fixative.
11.
If preferred method of collection cannot be done, scrapes should be
quickly smeared on one slide and immediately sprayed with Cytology
fixative. Two or three sprays are adequate. Hold spray bottle six inches
from the slide. Then proceed with the second slide.
12.
Please submit at least 2 slides from the lesion, if possible. When a
fungal lesion is suspected, one slide will be used for pap stain and
another for fungal stain.
13.
Allow fixed slides to dry completely before closing the cardboard slide
folder.
14.
Fold and wrap the Cytology form around the folder.
15.
Transport the smears and bottle of fixative directly to Cap-Lab
Cytology department. To arrange for transportation of specimens call
517-372-5520.
REFERENCE:
Naib, Zuher, M.D. : Exfoliative Cytopathology, Second Edition, Little,
Brown and Co., Boston, 1976.
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